Anatasya Putri Gusasi (1), Titania Rahma Aureria Inaku (2), Saputri Afra A. Pakaya (3), Nur Fadilah Sahran (4), Sintia ABD Rahman (5), Natasya Aprilia Putri Supardi (6)
Global population growth increases the demand for nutritious food, while tomato (Solanum lycopersicum L.) productivity often declines due to biotic and abiotic stress. CRISPR/Cas9 technology offers a precision approach to improving the nutritional quality, disease resistance, and metabolic efficiency of tomatoes. This review analyzes research from 2015 to 2025 on gene editing related to vitamin C enhancement, leaf morphology modification, fruit ripening, and strengthening resistance to pathogens such as TYLCV and powdery mildew, with Agrobacterium-based transformation proven effective in producing stable changes. However, challenges such as off-target risks and limited access to technology remain obstacles. Overall, CRISPR/Cas9 has the potential to accelerate tomato variety improvement for global food security, but further research is needed to enhance the precision and sustainability of its development.
Advenita, V. E. S. R., Mevotema, C., Situmorang, I. A., Haris, L., & Irawati, W. (2023). THE POTENCY OF VITAMIN C IN TOMATO PLANTS FOR THE RESULT OF GENETICALLY MODIFIED LANCEOLATE GENES THROUGH AGROBACTERIUM TUMEFACIENS USING CRISPR-CAS9. Journal of Tropical Biology.
Astuti, M. E., & Achamar, T. (2022). UTILIZATION OF TOMATOES BEYOND HOUSEHOLD CONSUMPTION IN EVERYDAY LIFE. Journal of Hulonthalo Service Society (JHSS), x(x), xx–xx. http://journal.ubmg.ac.id/index.php/JHSS
Carvalho Jr, A., Vicente, M. H., Ferigolo, L. F., Silva, E. M., Lira, B. S., Teboul, N., Levy, M., Bueno, S., Pereslazaro, E. P., Sablowski, R., Schommer, C., Valverde, F., Rossi, M., Ori, N., & Nogueira, F. T. (2024). THE MIR319?BASED REPRESSION OF SLTCP2/LANCEOLATE ACTIVITY IS REQUIRED FOR REGULATING TOMATO FRUIT SHAPE. The Plant Journal, 121(1), e17174.
Hasfikasari, P., Faradiba, F., & Amin, A. (2024). ANTIOXIDANT ACTIVITY OF TOMATO FRUIT EXTRACT (SOLANUM LYCOPERSICUM L.). Makassar Natural Product Journal, 2(1), 43–50.
Hermantara, R. (2024). CRISPR-CAS9: A STORY OF DISCOVERY, INNOVATION, AND REVOLUTION IN GENOME EDITING. Indonesian Journal of Life Sciences, 6(1), 83–104.
Kurniawati, D. A., Suharsono, & Santoso, T. J. (2020). PCNA GENE EDITING WITH CRISPR/CAS9 TECHNOLOGY TO IMPROVE CHILI PLANT RESISTANCE TO YELLOW CURLY LEAF DISEASE. Jurnal AgroBiogen, 16, 79–88. https://doi.org/10.21082/jbio.v16n2.2020.p79-88
Rao, M. J., & Wang, L. (2021). CRISPR/CAS9 TECHNOLOGY FOR IMPROVING AGRONOMIC TRAITS AND FUTURE PROSPECTIVE IN AGRICULTURE. Planta, 254, Article 68.
Roviati, E. (2022). GENETIC ENGINEERING IN TOMATOES. Academia, 3–6.
Rustan, M. N., Wahyuni, S., Baihaqi, B., Nafilawati, W. O., Ningsih, M. L., & Febryansyah. (2025). FOOD BIOTECHNOLOGY BASED ON GENETICALLY ENGINEERED MICROORGANISMS: GLOBAL TRENDS AND CHALLENGES. Jurnal Minfo Polgan, 13(2), 2521–2532.
Sari, L. D. A., Ningrum, R. S., Ramadhani, A. H., & Kurniawati, E. (2021). VITAMIN C CONTENT OF TOMATOES (LYCOPERSICUM ESCULENTUM MILL) AT EACH MATURITY PHASE BASED ON DAYS AFTER PLANTING. Indonesian Journal of Pharmacy and Pharmaceutical Sciences, 8(1), 78–82.
Santoso, T. J. (2018). CRISPR/CAS9 GENOME EDITING TECHNOLOGY FOR RICE IMPROVEMENT. Center for Agricultural Biotechnology and Genetic Resources Research and Development, 365.
Tiwari, J. K., Singh, A. K., & Behera, T. K. (2023). CRISPR/CAS GENOME EDITING IN TOMATO IMPROVEMENT: ADVANCES AND APPLICATIONS. Frontiers in Plant Science, 14, 1121209.
Yulianti, E., Pakaya, M. S., Yerizel, E., Septiprajaamalia, R. R., Uno, W. Z., Hasmiwati, H., Jannah, M., Arisanty, D., Slamet, R. A. W., Afriani, T., Maliza, R., Kustiawan, P. M., Bethasari, M., & Nurlila, R. U. (2024). GENETIC ENGINEERING. Purbalingga: CV Eureka Media